F4/80 antibody

Catalog No. ABIN111856

This Rat Monoclonal antibody specifically detects F4/80 in FACS, WB, IHC (p), IP, IHC (fro), RIA and Func. It exhibits reactivity toward Mouse.

Quick Overview for F4/80 antibody (ABIN111856)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This F4/80 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Functional Studies (Func)

Clone: Cl:A3-1

Product Details anti-F4/80 Antibody

Specificity: This antibody recognises the F4/80 antigen. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.

Purification: Affinity Chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Western Blot. Immunoprecipitation. Radioimmunoassays. Functional Assays. Flow Cytometry: Use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Resin Sections. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: Requires pre-treatment prior to staining.

Protocol: 1. Enzyme pre-treatment using Proteinase K (recommended for tissues fixed for 24 hoursin neutral buffered formalin, NBF): ReagentsA. TE buffer (50 mM Tris base, 1 mM EDTA, pH 8. 0)Tris Base, 6. 10 gEDTA, 0. 37 gDistilled water, 1000 ml

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, None, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: Macrophage F4/80 Antigen

Background: F4/80 is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

Gene ID: 13733

NCBI Accession: NP_034260

UniProt: Q61549