Fibroblasts (Pan Reticular) antibody

Antigen: Fibroblasts

Epitope: Pan Reticular

Clonality: Monoclonal (ER-TR7)

Host: Rat

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Catalog no.: ABIN111871

Additional Information

Characteristics: Synonyms: Fibroblast Marker, Fibroblasten

Alternative name: Fibroblasts (Pan Reticular)

Immunogen: Murine thymic reticulum. Epitope: The antigen has not been fully characterized, the epitope may be part of reticulin.

Isotype: IgG2a  (Matching secondary antibodies)

Clone: ER-TR7

Description: Connective tissue consists of a ground of glycosaminoglycans, proteoglycans andglycoproteins through which various fibres run. These fibres can be collagenous, elastic orreticular. Reticular fibres are composed from the family of collagen proteins and givetensile strength. These fibres are made by reticular fibroblasts.

Specificity: Reacts with Mouse reticular fibroblasts, reticular fibres and Human tonsil. ER-TR7 detects an antigen present in and produced by reticular fibroblasts. The recognizedantigen is most likely distinct from laminin, fibronectin, collagen types I-IV, heparansulfate proteoglycan, entactin, and nidogen. The antibody is useful to stain the microanatomy of various organs, in particular theconnective tissue framework in lymphoid organs. The antibody also stains subendothelialdeposits in the plaque area of atherosclerotic plaques. Antigen DistributionIsolated Cells: The antigen is found in the cytoplasm of fibroblast cell lines. Tissue Sections: The antigen is expressed in connective tissues which form a supporting

Application Details

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: (The whole procedure is performed at room temperature)1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti rat IgG (H+L)minimal-cross reaction to mouse) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayers hemalum

Application Notes: Immunofluorescence. Immunohistochemistry on Frozen Sections: 1 µg/ml (1/400) freshly prepared for Mousetissue and 4 µg/ml (1/100) on Human/Porcine tissue. Recommended Positive Control: Mouse spleen. Does not react on routinely processed Paraffin Sections. Has been described to work in FACS. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.4 mg/ml (after reconstitution)

Buffer: This stock solution contains PBS, pH 7.2 with 5 mg/ml BSA as a stabilizerand 0.09% Sodium Azide as preservative.Reconstitution: Restore with 0.5 ml distilled water.

Storage: Store the lyophilized antibody at 2-8°C. Freeze stock solution and aliquots thereof at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cell/Tissue Markers

Restrictions: For Research Use only

Images

Reactivity of ER-TR7 with various non-lymphoid organs of the mouse

Publications

Publications: Van Vliet, Melis, Foidart et al.: "Reticular fibroblasts in peripheral lymphoid organs identified by a monoclonal antibody." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 34, Issue 7, pp. 883-90, 1986 (PubMed).