Adeno-Associated Virus 2 (AAV-2) (VP1) antibody

Antigen: Adeno-Associated Virus 2 (AAV-2) (VP1)

Clonality: Monoclonal (A1)

Host: Mouse

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN112133

Additional Information

Characteristics: Synonyms: AAV2

Swiss-Prot: O56652

Immunogen: Adeno-associated virus capsid proteins and virus Particles.

Isotype: IgG2a  (Matching secondary antibodies)

Clone: A1

Specificity: A1 reacts with VP1 of adeno-associated virus. In immunoprecipitation, an occasionalreaction with a non-AAV-derived protein is found. Epitope mapping experiments (Wobus etal. , see below) identified aa123 to aa131 of VP1 capsid protein as the specific bindingregion. Species: Human, Monkey. Others not tested.

Application Details

Application Notes: Immunofluorescence microscopy. Immunohistochemistry: 1: 10 - 1: 20, 3 h at room temperature or overnight at 2-8C. Immunoprecipitation. Immunoblotting. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: Contains 0.09% sodium azide and 0.5% BSAReconstitution: Restore with 1 ml dist. water

Storage: Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Publications: Wistuba, Kern, Weger et al.: "Subcellular compartmentalization of adeno-associated virus type 2 assembly." in: Journal of virology, Vol. 71, Issue 2, pp. 1341-52, 1997 (PubMed).