Adeno-Associated Virus 2 (AAV-2) (VP1 / VP2) antibody

Antigen: Adeno-Associated Virus 2 (AAV-2) (VP1 / VP2)

Clonality: Monoclonal (A69)

Host: Mouse

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN112134

Additional Information

Characteristics: Synonyms: AAV2

Immunogen: Adeno-associated virus capsid proteins and virus particles.

Isotype: IgG1  (Matching secondary antibodies)

Clone: A69

Specificity: A69 reacts with VP 1 and VP 2 of adeno-associated virus which are highly enriched in thenucleus. Epitope mapping experiments (Wobus et al. , see references) identified aa169 toaa184 of VP2 and (with reduced intensity) aa123 to aa136 of VP1 capsid proteins as thespecific binding region. Weak cross-reaction with serotypes 1, 3, and 6Species: Human, Monkey. Others not tested.

Application Details

Application Notes: Western Blot. Immunofluorescence Microscopy. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/10. Incubation Time: 1h at RT. Dilution buffer: PBS or Tris-buffered saline. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS containing 0.5% BSA as stabilizer and 0.09% Sodium Azide aspreservative.Reconstitution: 1 ml distilled Water

Storage: Store the antibody at 2-8°C after reconstitution. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Publications: Wistuba, Weger, Kern et al.: "Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins." in: Journal of virology, Vol. 69, Issue 9, pp. 5311-9, 1995 (PubMed).

Wobus, Huegle-Doerr, Girod et al.: "Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection." in: Journal of virology, Vol. 74, Issue 19, pp. 9281-93, 2000 (PubMed).