Adeno-associated Virus (AAV) (VP1 + VP2 + VP3) antibody

Antigen: Adeno-associated Virus (AAV) (VP1 + VP2 + VP3)

Clonality: Monoclonal (B1)

Host: Mouse

Reactivity: Human, Monkey

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN112135

Additional Information

Characteristics: Synonyms: AAV2

Immunogen: Adeno-associated virus capsid proteins and virus particles.

Isotype: IgG1  (Matching secondary antibodies)

Clone: B1

Specificity: Useful for Immunolocalization studies of capsid formation in combination with monoclonalantibody A20 (Cat. No. BM5010). B1 reacts with free VP 1, VP 2 and VP 3 ofadeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in thenucleus and the cytoplasm. Epitope mapping experiments (Wobus et al. , see below) identified aa726 to aa733(C-terminus, common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection. Reacts with AAV-2, found in human and monkey, and all published AAV serotypes (AAV-4only weak cross-reaction). Species Reactivity: Tested: Human and Monkey.

Application Details

Application Notes: Immunofluorescence Microscopy. Immunoprecipitation (mainly free VP proteins). Immunohistochemistry on Frozen Sections (Fixation: Acetone or formaldehyde). Working Dilution: 1/10 with PBS or Tris-buffered saline. Incubation Time: 1h at RT. Immunoblotting (Western Blot). Affinity Chromatography. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody at 2-8°C after reconstitution. Shelf life: One year from despatch.

Restrictions: For Research Use only

Publications

Publications: Wistuba, Weger, Kern et al.: "Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins." in: Journal of virology, Vol. 69, Issue 9, pp. 5311-9, 1995 (PubMed).

Wistuba, Kern, Weger et al.: "Subcellular compartmentalization of adeno-associated virus type 2 assembly." in: Journal of virology, Vol. 71, Issue 2, pp. 1341-52, 1997 (PubMed).

Wobus, Huegle-Doerr, Girod et al.: "Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection." in: Journal of virology, Vol. 74, Issue 19, pp. 9281-93, 2000 (PubMed).