Desmoplakin antibody

Catalog No. ABIN112137

The Mouse Monoclonal anti-Desmoplakin antibody (Clone DP-2-17) (ABIN112137) specifically detects Desmoplakin in WB, IHC (fro) and IHC (p). The antibody is reactive with Human, Mouse, Rat, Chicken and Cow samples.

Quick Overview for Desmoplakin antibody (ABIN112137)

Target: Desmoplakin (DSP)

Reactivity: Human, Mouse, Rat, Chicken, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoplakin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DP-2-17

Product Details anti-Desmoplakin Antibody

Specificity: DP 2.17 shows distinct punctate membrane staining of epithelia. It reacts with the rod fragment of Desmoplakin 1 (Mr 250,000 polypeptide of desmosomal plaques). Tested Reactivities on Cultured Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, TR-146. Bovine cells: MDBK, BMGE.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Rat, Chicken and Mouse.

Purification: Protein A Affinity Chromatography

Immunogen: Bovine Desmoplakin 1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen and paraffin embedded sections after microwavetreatment (dilute 1: 10 with PBS pH 7.4). Western blot (Incubation time: 1h at RT).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Expiry Date: 6 months

Target Details for Desmoplakin

Target: Desmoplakin (DSP)

Alternative Name: Desmoplakin

Background: Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.Synonyms: 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP

Gene ID: 1832

NCBI Accession: NP_001008844

UniProt: P15924

Pathways: Cell-Cell Junction Organization