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Desmoplakin antibody

DSP Reactivity: Human, Mouse, Rat, Cow, Chicken WB, IHC (fro), IHC (p) Host: Mouse Monoclonal DP-2-17 unconjugated
Catalog No. ABIN112137
  • Target See all Desmoplakin (DSP) Antibodies
    Desmoplakin (DSP)
    Reactivity
    • 33
    • 16
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Cow, Chicken
    Host
    • 28
    • 4
    • 2
    Mouse
    Clonality
    • 30
    • 4
    Monoclonal
    Conjugate
    • 23
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Desmoplakin antibody is un-conjugated
    Application
    • 15
    • 11
    • 11
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    DP 2.17 shows distinct punctate membrane staining of epithelia. It reacts with the rod fragment of Desmoplakin 1 (Mr 250,000 polypeptide of desmosomal plaques). Tested Reactivities on Cultured Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, TR-146. Bovine cells: MDBK, BMGE.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Bovine, Rat, Chicken and Mouse.
    Purification
    Protein A Affinity Chromatography
    Immunogen
    Bovine Desmoplakin 1
    Clone
    DP-2-17
    Isotype
    IgG1
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    Discover our top product DSP Primary Antibody
  • Application Notes
    Immunohistochemistry on frozen and paraffin embedded sections after microwavetreatment (dilute 1: 10 with PBS pH 7.4). Western blot (Incubation time: 1h at RT).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1 mL distilled water.
    Buffer
    PBS, pH 7.4 containing 0.09 % Sodium Azide and 0.5 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
    Expiry Date
    6 months
  • Target
    Desmoplakin (DSP)
    Alternative Name
    Desmoplakin (DSP Products)
    Synonyms
    DP antibody, DPI antibody, DPII antibody, DSP antibody, dpi antibody, dpii antibody, 2300002E22Rik antibody, 5730453H04Rik antibody, AA407887 antibody, AA407888 antibody, AW109828 antibody, im:6911953 antibody, sb:cb570 antibody, wu:fc17a08 antibody, wu:fk73d01 antibody, desmoplakin antibody, Desmoplakin antibody, ORF113 antibody, desmoplakin a antibody, ORF112 DESMOPLAKIN antibody, orf102 antibody, orf91; similar to Cydia pomonella granulovirus orf112 antibody, ORF62 antibody, ORF-66 antibody, ORF57 antibody, agip105 antibody, similar to XcORF133; similar to HaGV desmoplakin antibody, desmoplakin L homeolog antibody, ORF96 antibody, DSP antibody, PhopGV104 antibody, ORF-59 desmoplakin antibody, desmop antibody, Dsp antibody, dsp antibody, desmoplakin antibody, dspa antibody, orf112 desmoplakin antibody, ClgVgp102 antibody, COGV_gp091 antibody, APNV_p087 antibody, CBNV_gp066 antibody, EONV_gp057 antibody, OLNV_gp066 antibody, AgipMNPV_gp105 antibody, HaMNV_gp106 antibody, EupsNPV_gp065 antibody, PsunGV_gp139 antibody, dsp.L antibody, ClanGV_gp096 antibody
    Background
    Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.Synonyms: 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP
    Gene ID
    1832
    NCBI Accession
    NP_001008844
    UniProt
    P15924
    Pathways
    Cell-Cell Junction Organization
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