C1q antibody

Antigen: C1q

Clonality: Monoclonal (242G3)

Host: Mouse

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Functional Studies (Func), Western Blotting (WB)

Catalog no.: ABIN112267

Additional Information

Characteristics: Synonyms: Complement C1q, Complement 1q

Swiss-Prot: P02745

Immunogen: Purified human complement C1q.

Isotype: IgG1  (Matching secondary antibodies)

Clone: 242G3

Description: C1q is a sub-component of the first complement component C1, which is formed by theinteraction of C1q with the tetramer C1s-C1r-C1r-C1s. C1q is a member of a family of soluble collagen-like proteins and is composed of threebasic subunits termed A, B and C (27,550, 25,200, 23,800 Da respectively) which asociatein the proportion 6 A-B subunits (52, 750 Da) and 3 C-C subunits (47,600 Da), to formwhole C1q (459,300 Da). C1q binds to IgG and IgM immune complexes, this activates C1rand C1s, which are also bound to C1q and so initiates the classical pathway of complementactivation.

Specificity: Detection of C1q in serum, in serum-C1 and within immune complexes, inhibits hemolyticactivity of C1 by binding to C1q in the liquid phase (antibody concentration necessary toinhibit 50% of hemolytic activity in the fluid phase is 0. 85 µg/ml). With C1 bound toimmune complexes a dose dependent increase of C1 activity was observed at antibodyconcentrations of 1. 5 µg/ml (cf. ref. 1). Useful for detection of C1q-bearing immune complexes in patients with rheumaticdisorders. Polypetide Reacting: C-chain of complement C1q (25 kD in SDS-PAGE). Species: Human. Others not tested.

Application Details

Application Notes: ELISA. Inhibition experiments (e. g. hemolytic activity). Immunoblotting (Western): 1/100, 1h at RT (ECL method). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: Final Solution contains PBS buffer, pH 7.4 with 0.09% Sodium Azide aspreservative and 0.5% BSA as stabilizer.Reconstitution: Restore in 1 ml distilled water.

Storage: Store the antibody at 2-8°C. Shelf life: one year from despatch.

Research Area: Neurology, Innate Immunity, Complement Factors

Restrictions: For Research Use only

Publications

Publications: Antes, Heinz, Loos: "Detection of C1q-bearing immune complexes by a monoclonal anti-C1q ELISA system." in: Journal of immunological methods, Vol. 102, Issue 2, pp. 149-56, 1987 (PubMed).

Antes, Heinz, Loos: "Enzyme-linked immunosorbent assay for C1q in human serum by use of monoclonal antibodies." in: Journal of immunological methods, Vol. 74, Issue 2, pp. 299-306, 1985 (PubMed).

Heinz, Burger, Golan et al.: "Activation of the first component of complement, C1, by a monoclonal antibody recognizing the C chain of C1q." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 2, pp. 804-8, 1984 (PubMed).