Cadherin 6 antibody

Antigen: Cadherin 6

Synonyms: cad6, MGC129338, MGC129360, K-cadherin, cadherin-6

Clonality: Monoclonal (2B6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN112365

Additional Information

Characteristics: Synonyms: CDH6, Kidney cadherin, Cadherin K, K-Cadherin

Alternative name: Cadherin-6

Swiss-Prot: P55285

Immunogen: Affinity purified extracellular domain of human cadherin-6-GST fusion protein

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2B6

Description: Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+dependentcell-cell interactions. The members of this family are differentially expressed invarious tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and acytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins whichalso function as signal transducers. The extracellular domains are responsible for thespecificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelialcadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin orcadherin-2), placental cadherin(P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin(K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type IIcadherins. Cadherin-6 is expressed in the proximal tubule epithelium of the kidney fromwhich most renal cell carcinomas are derived.

Specificity: Clone 2B6 recognizes the extracellular domain of cadherin-6. Species: Human, rat. Others not tested.

Application Details

Application Notes: Immunoblotting (1: 25 - 1: 250). Immunocytochemistry. Immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mMCaCl2 and 0.1 mM MgCl2. Recommended range is 1: 25 - 1: 50 for immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 0.09% sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Extracellular Matrix, Signaling, Cytoskeleton

Restrictions: For Research Use only

Publications

Publications: Shimazui, Oosterwijk, Akaza et al.: "Expression of cadherin-6 as a novel diagnostic tool to predict prognosis of patients with E-cadherin-absent renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 4, Issue 10, pp. 2419-24, 1998 (PubMed).

Shimazui, Oosterwijk-Wakka, Akaza et al.: "Alterations in expression of cadherin-6 and E-cadherin during kidney development and in renal cell carcinoma." in: European urology, Vol. 38, Issue 3, pp. 331-8, 2000 (PubMed).