Selectin E (SELE) antibody

Antigen: Selectin E (SELE)

Synonyms: ELAM, ESEL, CD62E, ELAM1, LECAM2, SELE

Clonality: Monoclonal (UZ4)

Host: Rat

Reactivity: Human, Mouse (Murine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Functional Studies (Func), Western Blotting (WB)

Catalog no.: ABIN112379

Additional Information

Characteristics: Synonyms: SELE, ELAM1, LECAM2, Endothelial leukocyte adhesion molecule 1, Leukocyte-endothelialcell adhesion molecule 2, CD62 antigen-like family member E

Alternative name: CD62E / E-Selectin

Swiss-Prot: P16581

Immunogen: LPS-activated mlEND1 cells expressing E-selectin at their cell surface

Isotype: IgM  (Matching secondary antibodies)

Clone: UZ4

Description: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillaryvenules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries andsmall venules at any site in the body after onset of inflammation. This response isimmediate and involves a cascade of adhesion receptors. At the endothelial surfacemembers of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the bloodvessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments haveshown that IL-1, TNF-alpha and bacterial wall components like lipopolysaccharides inducethe transcription of E-selectin in a NFkB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatorysituations.

Specificity: UZ4 recognizes the minimal amino acid consensus sequence CXKKKL present in the lectindomain of both murine and human E-selectin. Species: Human, mouse.

Application Details

Application Notes: UZ4 is a functional antibody in that it inhibits leukocyte adhesion to both murine andhuman activated endothelial cells. For in vivo application in mice 20 g of purified Ig perinjection is recommended. UZ4 is also suitable for immunoblotting, ELISA, immunoprecipitation and flow cytometry(dilution 1: 500). It is not optimal for immunohistochemistry, to analyze mouse E-selectin protein in frozensections, please refer to the anti E-selectin monoclonal antibodies UZ5 (ABIN112380), UZ6(ABIN112381), and UZ7 (ABIN112382). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 0.09% sodium azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Hallmann, Zimmermann, Sorokin et al.: "Adhesion of leukocytes to the inflammed endothelium." in: Scandinavian journal of rheumatology. Supplement, Vol. 101, pp. 107-9, 1995 (PubMed).

Austrup, Vestweber, Borges et al.: "P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues." in: Nature, Vol. 385, Issue 6611, pp. 81-3, 1997 (PubMed).

Borges, Tietz, Steegmaier et al.: "P-selectin glycoprotein ligand-1 (PSGL-1) on T helper 1 but not on T helper 2 cells binds to P-selectin and supports migration into inflamed skin." in: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 573-8, 1997 (PubMed).

Tietz, Allemand, Borges et al.: "CD4+ T cells migrate into inflamed skin only if they express ligands for E- and P-selectin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 2, pp. 963-70, 1998 (PubMed).

Alferink, Tafuri, Vestweber et al.: "Control of neonatal tolerance to tissue antigens by peripheral T cell trafficking." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1338-41, 1998 (PubMed).

Wolf, Hallmann, Sass et al.: "TNF-alpha-induced expression of adhesion molecules in the liver is under the control of TNFR1--relevance for concanavalin A-induced hepatitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 2, pp. 1300-7, 2001 (PubMed).

Hammel, Weitz-Schmidt, Krause et al.: "Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion." in: Experimental cell research, Vol. 269, Issue 2, pp. 266-74, 2001 (PubMed).

Sunderkoetter, Seeliger, Schoenlau et al.: "Different pathways leading to cutaneous leukocytoclastic vasculitis in mice." in: Experimental dermatology, Vol. 10, Issue 6, pp. 391-404, 2001 (PubMed).