COL3A1 antibody (Chain alpha 1)

Catalog No. ABIN112535

The Mouse Monoclonal anti-COL3A1 antibody is suitable to detect COL3A1 in samples from Human. It has been validated for IF, IHC (p), EIA, IHC (fro) and RIA.

Quick Overview for COL3A1 antibody (Chain alpha 1) (ABIN112535)

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This COL3A1 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Clone: 8D1-8C7

Product Details anti-COL3A1 Antibody

Binding Specificity: Chain alpha 1

Specificity: Human Collagen type III 100 % (solid phase RIA at 1/500 dilution). Type III Collagen, MW 95 kDa, can be observed in skin, cartilage, human placenta and vitreous body. Cross Reactions: Human Collagen I, II, IV and V: < 0,1 % Human Fibronectin and Laminin: < 0,1 %. Does not react with Mouse, Rat, Bovine and Pig.

Purification: Affinity Chromatography.

Immunogen: Purified collagen type III from human placenta

Isotype: IgG1

Application Details

Application Notes: ELISA. RIA (> 1/1000). Immunflourescence assay: 1/80Immunohistochemistry on Paraffin Sections: 1/1000 (60 min at RT or 2-8 °C over night). Immunohistochemistry on Frozen Sections. Pretreatment for IHC: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app.300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding isblocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 %peroxide solution in TBS for 30 min at RT is recommended. Positive Control: Human skin and placenta.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.1 mL distilled water.

Concentration: 1.0 mg/mL

Buffer: PBS without preservatives or BSA.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Target Details for COL3A1

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Background: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage, vitreous body, and human placenta.Synonyms: COL3A1

Gene ID: 1281

NCBI Accession: NP_000081

UniProt: P02461

Pathways: Autophagy, Growth Factor Binding