ENO1 antibody

Catalog No. ABIN112663

This Rabbit Polyclonal antibody specifically detects ENO1 in WB, IHC (p), EIA and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for ENO1 antibody (ABIN112663)

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ENO1 Antibody

Specificity: This antibody detects Human Non-Neuronal Enolase, and demostrates less than 0.1 % cross-reactivity with non-specific Enolase.

Purification: Affinity Chromatography on Protein G

Immunogen: Native Human NNE.

Application Details

Application Notes: Immunohistochemistry on Frozen and Paraffin Sections (each 1/25-1/100). ELISA. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.0 mg/mL (by OD 280 nm)

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: alpha-Enolase (ENO1)

Background: Non Neuronal Enolase (ENO1) is an isoform of mammalian enolase and is composed of 2 alpha subunits. The gene for ENO1 encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. Non Neuronal Enolase can be as a diagnostic marker for many tumors, as a marker for endometriosis and in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. It has been identified as an autoantigen in Hashimoto encephalopathy (associated with Hashimoto thyroiditis).Synonyms: 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, ENO1L1 Non-neural enolase, Enolase 1, MBP-1, MBPB1, MPB-1, MPB1, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein

Gene ID: 2023

NCBI Accession: NP_001188412

UniProt: P06733