CD8 antibody

Catalog No. ABIN114167

The Rat Monoclonal anti-CD8 antibody has been validated for FACS and IHC (fro). It is suitable to detect CD8 in samples from Mouse.

Quick Overview for CD8 antibody (ABIN114167)

Target: CD8

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: YTS169-4

Product Details anti-CD8 Antibody

Specificity: This clone recognises the murine CD8 cell surface antigen, expressed by a subset of T lymphocytes.

Purification: Affinity chromatography on Protein G

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry (1: 50 - 1: 100, use 10 μL to label 10^6 cells in 100 μL. Method sheets areavailable on request). Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline pH 7.4, containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD8

Target: CD8

Alternative Name: CD8

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID: 12525

NCBI Accession: NP_001074579

UniProt: P01731