CD8 antibody (FITC)

Catalog No. ABIN135497

Product Details anti-CD8 Antibody (FITC)

Target: CD8

Reactivity: Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Feline/Lion/Tsushima Leopard Cat CD8β, Mr 31 & 38 kDa

Characteristics: Mouse Anti-Feline CD8-FITC

Purification: Protein A

Immunogen: Feline thymocytes

Clone: FCD8

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Sep - Reported in literature
• Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-CD8 antibody (FITC) (ABIN135497)

Carballeda, Zoth, Gómez, Gravisaco, Berinstein: "Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain." in: Immunobiology, Vol. 216, Issue 9, pp. 1028-33, (2011) (PubMed).

Target Details for CD8

Target: CD8

Alternative Name: CD8 (CD8 Products)

Background: Feline CD8, a member of the immunoglobulin superfamily of cell surface receptors, is a type II transmembrane glycoprotein that is expressed as a heterodimer on the suppressor/cytotoxic subpopulation of peripheral T lymphocytes. It is present on approximately 63 % of thymocytes, 9 % of splenocytes, 20 % of lymph node cells, and 15 % of peripheral blood lymphocytes. CD8 functions as a co-receptor with MHC Class I-restricted T cell receptors in antigen recognition.