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- Target See all Neutrophils products
- Neutrophils
- Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This Neutrophils antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Characteristics
- Absorption / Emission: 488 nm / 575 nm
- Purification
- Protein A affinity purified Ig
- Immunogen
- Cultured bone marrow cells
- Clone
- 07-04-14
- Isotype
- IgG2a
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anti-Neutrophils antibody (PE)
Reactivity: Mouse FACS Host: Rat Monoclonal 07-04-14 PE
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- Application Notes
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Flow cytometry (see protocol).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation using an NH4Cl lysing buffer.
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS with 0.09 % NaN3 as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
- Expiry Date
- 6 months
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- Target
- Neutrophils
- Abstract
- Neutrophils Products
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