H-2Dd antibody (Biotin)

Catalog No. ABIN114287

The Mouse Monoclonal anti-H-2Dd antibody is suitable to detect H-2Dd in samples from Mouse. It has been validated for FACS.

Quick Overview for H-2Dd antibody (Biotin) (ABIN114287)

Target: H-2Dd (MHC Class I H-2Dd)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H-2Dd antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 34-5-8S

Product Details anti-H-2Dd Antibody (Biotin)

Specificity: This mAb is specific for cells expressing the H-2D antigen coded for by the d haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody detects a private determinant (H-2.4) of the H-2Dd antigen. This antibody can be used to quantitate cells bearing H-2Dd (H-2.4) antigen from the appropriate strains of mice.

Purification: Protein G Chromatography

Immunogen: B6xDBA/2 spleen cells Donor: C3H/HeJ spleen Fusion Partner: SP2/0.Ag14

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method:

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS , 0.02 % NaN3 and EIA grade BSA as a stabalizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for H-2Dd

Target: H-2Dd (MHC Class I H-2Dd)

Alternative Name: MHC Class I H-2 Dd

Background: Synonyms: D-D alpha chain, H-2 class I histocompatibility antigen, H-2D(D), H2-D1

Gene ID: 100045864

NCBI Accession: XP_003086970

UniProt: P01900