MHC Class I (H-2Kb/H-2Db) antibody

Catalog No. ABIN114293

The Mouse Monoclonal anti-MHC Class I (H-2Kb/H-2Db) antibody is suitable to detect MHC Class I (H-2Kb/H-2Db) in samples from Mouse. It has been validated for CyTox.

Quick Overview for MHC Class I (H-2Kb/H-2Db) antibody (ABIN114293)

Target: MHC Class I (H-2Kb/H-2Db)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Cytotoxicity Test (CyTox)

Clone: 5041-16-1

Product Details

Purification: Protein G Chromatography

Isotype: IgG2a

Application Details

Application Notes: Cytotoxicity assays. Functional testing. (For details please see Protocols below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF H-2Kb AND/OR H-2DbBEARING LYMPHOCYTES: 1. Prepare a cell suspension from the appropriate tissue in a cytotoxicity medium (1) orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on a density cell separation medium (2). After washing, adjust the cell concentration to 1x10e7 cells per ml in cytotoxicity medium. 2. Add the antibody to a final concentration of 1: 100 and mix. Alternatively, pellet the cellsand resuspend in antibody diluted 1: 100 in cytotoxicity medium. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant5. Resuspend to the original volume in a low-toxicity rabbit complement (3), diluted to theappropriate concentration in cytotoxicity medium. 6. Incubate for 60 minutes at 37°C. 7. Monitor for percent cytotoxicity at this stage, before further processing. For this purpose

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, without preservative

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: MHC Class I (H-2Kb/H-2Db)

Alternative Name: MHC Class I H-2 Kb / H-2 Db

Background: Synonyms: HLA Class I