MHC Class I (H-2Kb/H-2Db) antibody (Biotin)

Catalog No. ABIN114294

The Biotin-conjugated Mouse Monoclonal anti-MHC Class I (H-2Kb/H-2Db) antibody (Clone 5041-16-1) (ABIN114294) specifically detects MHC Class I (H-2Kb/H-2Db) in FACS. The antibody is reactive with Mouse samples.

Quick Overview for MHC Class I (H-2Kb/H-2Db) antibody (Biotin) (ABIN114294)

Target: MHC Class I (H-2Kb/H-2Db)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Clone: 5041-16-1

Product Details

Purification: Affinity Chromatography on Protein A.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry (see Protocol below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSISMethod: 1. Prepare a cell suspension in media A. For spleen cell preparations, deplete the red blood

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg IgG /ml

Buffer: PBS containing 0.02 % Sodium Azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: MHC Class I (H-2Kb/H-2Db)

Alternative Name: MHC Class I H-2 Kb / H-2 Db

Background: Synonyms: HLA Class I