MHC Class II I-Ek antibody (Biotin)

Catalog No. ABIN114317

The Mouse Monoclonal anti-MHC Class II I-Ek antibody is suitable to detect MHC Class II I-Ek in samples from Mouse. It has been validated for FACS.

Quick Overview for MHC Class II I-Ek antibody (Biotin) (ABIN114317)

Target: MHC Class II I-Ek

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II I-Ek antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 14-4-4S

Product Details anti-MHC Class II I-Ek Antibody (Biotin)

Purification: Protein G Chromatography

Immunogen: C3H Donor: C3H.SW Fusion Partner: SP2/0-Ag 14

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MHC Class II I-Ek

Target: MHC Class II I-Ek