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Keratin 20 (KRT20) antibody

Details for Product No. ABIN114613
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Antigen
Synonyms KRT20, CD20, CK-20, CK20, K20, KRT21, Krt21, 9030623C06Rik
Reactivity
Human
(150), (39), (37), (5), (1)
Host
Mouse
(78), (76), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(115), (75), (50), (36), (22), (15), (12), (10), (4), (3), (3), (2)
Pubmed 4 references available
Quantity 0.5 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN114613
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Immunogen Cytokeratins isolated from human micro dissected villi of human duodenal mucosa.
Clone Ks20-8
Isotype IgG2a
Specificity This antibody reacts with cytokeratin polypeptide of 46 kD in immunoblott analyses. Cellular Localization: Cytoplasmic.
Purification Purified
Alternative Name Cytokeratin 20
Background Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas. This antibody is essentially non-reactive in squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas. This antibody is often used in conjunction with CK7 and other antibodies to distinguishing colon carcinomas (CK20+), from ovarian, pulmonary, and breast carcinomas.
Alternate names: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
Gene ID 54474
NCBI Accession NP_061883.1
UniProt P35900
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer
Application Notes Immunohistochemistry on Paraffin Sections: Use a dilution of 1/25-1/50 in an ABC method(30 minutes at room temperature). Formalin fixed paraffin embedded tissue sectionsrequire high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior toimmunostaining. Positive Control: Skin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Supplier Images
anti-Keratin 20 (KRT20) antibody Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 20 antibody
General Takahashi, Fujita, Okabe et al.: "Immunohistochemical characterization of basal cell adenomas of the salivary gland." in: Pathology, research and practice, Vol. 187, Issue 2-3, pp. 145-56, 1991 (PubMed).

Angus, Kiberu, Purvis et al.: "Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1." in: The Journal of pathology, Vol. 155, Issue 1, pp. 71-5, 1988 (PubMed).

Gatter, Abdulaziz, Beverley et al.: "Use of monoclonal antibodies for the histopathological diagnosis of human malignancy." in: Journal of clinical pathology, Vol. 35, Issue 11, pp. 1253-67, 1983 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Validation Images
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