Keratin 20 (KRT20) antibody

Antigen: Keratin 20 (KRT20)

Synonyms: K20, CD20, CK20, KRT21, MGC35423, 9030623C06Rik, Krt21, KRT20, MGC152269

Clonality: Monoclonal (Ks20-8)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN114613

Additional Information

Alternative name: Cytokeratin 20

UniProt: P35900

Immunogen: Cytokeratins isolated from human micro dissected villi of human duodenal mucosa.

Cross-Reactivity: Human

Isotype: IgG2a

Clone: Ks20-8

Description: Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restrictedprimarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibodyis highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colonstomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors,transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas. This antibody is essentially non-reactive in squamous cell carcinomas andadenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of theovary, and small cell carcinomas. This antibody is often used in conjunction with CK7 andother antibodies to distinguishing colon carcinomas (CK20+), from ovarian, pulmonary,and breast carcinomas.

Characteristics: Synonyms: Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT, Keratin type I cytoskeletal 20

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: Use a dilution of 1/25-1/50 in an ABC method(30 minutes at room temperature). Formalin fixed paraffin embedded tissue sectionsrequire high temperature antigen unmasking with 10 mM Citrate buffer, pH 6.0 prior toimmunostaining. Positive Control: Skin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 20 antibody ABIN114612/ABIN114612-05

Publications

Takahashi, Fujita, Okabe et al.: "Immunohistochemical characterization of basal cell adenomas of the salivary gland." in: Pathology, research and practice, Vol. 187, Issue 2-3, pp. 145-56, 1991 (PubMed).

Angus, Kiberu, Purvis et al.: "Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1." in: The Journal of pathology, Vol. 155, Issue 1, pp. 71-5, 1988 (PubMed).

Gatter, Abdulaziz, Beverley et al.: "Use of monoclonal antibodies for the histopathological diagnosis of human malignancy." in: Journal of clinical pathology, Vol. 35, Issue 11, pp. 1253-67, 1983 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).