Thymidine Phosphorylase antibody

Catalog No. ABIN114826

This Mouse Monoclonal antibody specifically detects Thymidine Phosphorylase in WB and IHC (p). It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Thymidine Phosphorylase antibody (ABIN114826)

Target: Thymidine Phosphorylase (TYMP)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thymidine Phosphorylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: P-GF-44C

Product Details anti-Thymidine Phosphorylase Antibody

Specificity: This antibody is specific to a 55 kDa protein known as Platelet-Derived Endothelial Growth Factor (PD-ECGF), similar to thymidine phosphorylase (TP). Cellular localization: Cytoplasmic, Nuclear.

Purification: Purified

Immunogen: A recombinant full length human thymidine phophorylase (TP/PD-ECGF) protein.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of1/25-1/75 in an ABC method (30 minutes at room temperature). Western blotting: 1/50-1/100. Recommended Positive Control: Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Background: Platelet - derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites. High levels of TP/PD-ECGF are observed in cancer patients. High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.Synonyms: ECGF1, Gliostatin, PD-ECGF, Platelet-derived endothelial cell growth factor, TdRPase

Gene ID: 1890

NCBI Accession: NP_001107227

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2