Rabbit anti-Human IgG Antibody

Catalog No. ABIN115322

Quick Overview for Rabbit anti-Human IgG Antibody (ABIN115322)

Target: IgG

Clonality: Polyclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Reactivity: Human

Host: Rabbit

Purification: Purified

Immunogen: IgG isolated from a pool of normal Human sera.

Application Details

Application Notes: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at1/200-1/500 dilution in an ABC method (30 minutes at room temperature). Proteolytic treatment is required prior to immunostaining of formalin fixed paraffinembedded tissue sections. Recommended Positive Control: Tonsil

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 18.9 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgG

Target Type: Antibody

Background: IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75 % of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66 %), IgG2 (23 %), IgG3 (7 %) and IgG4 (4 %).Synonyms: Human Immunoglobulin G