alpha-1-Chymotrypsin antibody

Antigen: alpha-1-Chymotrypsin

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Catalog no.: ABIN115360

Additional Information

Alternative name: Alpha-1-chymotrypsin (ACT)

Gene ID: 12

UniProt: P01011

Immunogen: Alpha-1-antichymotrypsin isolated from human serum

Cross-Reactivity: Human

Description: Alpha 1 Antichymotrypsin is a plasma protease inhibitor and member of the serineprotease inhibitor class. Polymorphisms in this protein appear to be tissue specific andinfluence protease targeting. Variation in this protein's sequence have been implicated inAlzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructivepulmonary disease.

Characteristics: Synonyms: SERPINA3, AACT, GIG24, GIG25, Cell growth-inhibiting gene 24/25 protein

Specificity: The antibody is specific against human alpha-1-antichymotrypsin. Cellular Localization: Cytoplasmic. Species: Human. Others not tested. Add. Information: This antibody shows cross reactivity against wide range of mammalian species.

Application Details

Application Notes: Immunohistochemistry on frozen sections. Immunohistochemistry on formalin-fixed paraffin embedded sections: diluted of 1: 25-1: 50in an ABC method (30 minutes at room temperature). Recommended Positive Control: Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: Containing sodium azide as preservative

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Ropp, Copeland: "Cloning and characterization of the human mitochondrial DNA polymerase, DNA polymerase gamma." in: Genomics, Vol. 36, Issue 3, pp. 449-58, 1997 (PubMed).

Copeland: "Expression, purification, and characterization of the two human primase subunits and truncated complexes from Escherichia coli." in: Protein expression and purification, Vol. 9, Issue 1, pp. 1-9, 1997 (PubMed).