Interferon gamma antibody

Catalog No. ABIN115857

The Rabbit Polyclonal anti-Interferon gamma antibody (ABIN115857) specifically detects Interferon gamma in WB, Func and EIA. The antibody is reactive with Mouse samples.

Quick Overview for Interferon gamma antibody (ABIN115857)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Product Details anti-Interferon gamma Antibody

Specificity: Detects Interferon gamma (IFNg).

Purification: Affinity Chromatography employing immobilized Murine IFN-gamma matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Murine IFN-gamma Remarks: Molecular Weight: ~150 kDa

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofMurine IFN-gamma (1.00 ng/mL), a concentration of 0.05-0.1 μg/mL of this antibody isrequired. Indirect ELISA To detect mIFN-gamma by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof at least 0.2-0.4 ng/well of recombinant mIFN-gamma. Sandwich ELISA: To detect mIFN-gamma by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with biotinylated anti-murine IFN-gamma (PP004B1/B2)as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinantmIFN-gamma. Western Blot: To detect mIFN-gamma by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIFN-gamma is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without additives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at -20 °C for one year from despatch. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for six months when stored at -20 °C.

Expiry Date: 12 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma

Background: Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN

Gene ID: 15978

NCBI Accession: NP_032363

UniProt: P01580

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy