Cyclin T1 antibody (Internal Region)

Catalog No. ABIN116513

The Rabbit Polyclonal anti-Cyclin T1 antibody has been validated for WB, IP, IHC (p), EIA and IHC (fro). It is suitable to detect Cyclin T1 in samples from Human, Mouse and Rat.

Quick Overview for Cyclin T1 antibody (Internal Region) (ABIN116513)

Target: Cyclin T1 (CCNT1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin T1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cyclin T1 Antibody

Binding Specificity: Internal Region

Specificity: Antiserum will specifically react with a 85 kDa cyclin T1 protein. No reaction was observed against other related cyclins.

Purification: Delipidation and defibrination

Immunogen: Cyclin T1 peptide corresponding to an internal region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: Suitable for ELISA, immunoprecipitation, immunohistochemistry, immunoblotting andother immunological methods requiring high titer and specificity. Recommended dilutions: ELISA 1: 1,000 - 1: 5,000Western blot 1: 500 - 1: 2,000Immunohistochemistry 1: 200 - 1: 1,000 (use paraffin embedded tissue).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 75 mg/mL (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, containing 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C to -80 °C. Avoid multiple freeze/thaw cycles.

Target Details for Cyclin T1

Target: Cyclin T1 (CCNT1)

Alternative Name: Cyclin T1

Background: Cyclin T1, together with the kinase CDK9, is a component of the transcription elongation factor P-TEFb which binds the human immunodeficiency virus type 1 (HIV-1) transactivator Tat. Tat stimulates human HIV-1 viral transcription elongation. This suggests that cyclin T1/cdk9(PITALRE) is one of the HIV-1 required host cellular cofactors generated during T cell activation. Cyclin T1/cdk9(PITALRE) is shown to interact with Tat to restore Tat activation in HeLa nuclear extracts depleted of P-TEFb. P-TEFb facilitates transcription by phosphorylating the carboxy-terminal domain (CTD) of RNA polymerase II. The cdk9(PITALRE) activity and cyclin T1 are essential for activation of transcription when tethered to the heterologous Rev response element RNA via the regulator of expression of virion Rev. Cyclin T1 is an exceptionally large cyclin and is therefore a candidate for interactions with regulatory proteins.Synonyms: CCNT1, CycT1, Cyclin-T, Cyclin-T1

Gene ID: 904

NCBI Accession: NP_001231

UniProt: O60563