CIITA antibody (C-Term)

Catalog No. ABIN116528

The Rabbit Polyclonal anti-CIITA antibody is suitable to detect CIITA in samples from Human. It has been validated for WB, IP and EIA.

Quick Overview for CIITA antibody (C-Term) (ABIN116528)

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CIITA antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-CIITA Antibody

Binding Specificity: AA 726-742, C-Term

Sequence: CGEIKDNKEL PQYLALTTR

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Cross reactivity against CIITA from other sources may occur but has not been specifically determined.

Purification: Multi-step process.

Immunogen: Peptide corresponding to a region near the C-terminal end (amino acids 726-742) of the human CIITA gene conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed by immunoblot against CIITAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 is suggested for this product. Best Results are obtained using 0.03 % (v/v) Triton X-100 IN BUFFERS. Do not supplement buffers with Tween-20 detergent.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store original vial and reconstituted antibody at -20 °C.

Target Details for CIITA

Target: CIITA (Class II, Major Histocompatibility Complex, Transactivator (CIITA))

Alternative Name: CIITA / C2TA

Background: The transactivator CIITA regulates basal and interferon-induced expression of MHC class II genes. CIITA restores expression of all MHC II gene expression in mutant cells and corrects regulatory defects of those genes. Activation is via the proximal promoter. No DNA binding of in vitro translated CIITA was detected. CIITA may act in a coactivator-like fashion through protein-protein interactions by contacting factors binding to the proximal MHC class II promoter, to elements of the transcription machinery, or both. Alternatively it may activate HLA class II transcription by modifying proteins that bind to the MHC class II promoter.Synonyms: MHC class II transactivator, MHC2TA

Gene ID: 4261

NCBI Accession: NP_000237

UniProt: P33076

Pathways: Cancer Immune Checkpoints