Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A) antibody

Antigen: Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A)

Synonyms: ADH1, Adh-1, ADH-AA, Adh-1e, Adh-1t, Adh-3e, Adh1-e, Adh1-t, Adh1tl, Adh3-e, Adh-1-t, AI194826, Adh, Adh1a, ADH, ALCOHOL DEHYDROGENASE, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, ADC1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1, DKFZp470F1127

Clonality: Polyclonal

Host: Rabbit

Reactivity: Yeast (Pichia pastoris)

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116588

Additional Information

Characteristics: Synonyms: Alcohol dehydrogenase I, YADH-1, ADH1, ADC1, YOL086C, O0947

Alternative name: Alcohol dehydrogenase 1

Swiss-Prot: P00330

Immunogen: Full length Alcohol Dehydrogenase isolated from yeast.

Format: Serum

Description: Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion ofacetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety ofprimary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcoholdehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur atpositions 137, 138, 242-244, and 255 and near position 287.

Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified andpartially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due tosequence homology as determined by BLAST analysis. Cross reactivity with Alcohol Dehydrogenase from other sources is unknown. Add. Information: Protein Sequence: Yeast Alcohol Dehydrogenase, 347 aa, predicted MW 36. 7 kDa1 mtipdkqlaa vfhthggpen vkfeevpvae pgqdevlvni kytgvchtdl halqgdwplp61 akmpligghe gagvvvkvga gvtrlkigdr vgvkwmnssc gnceycmkae eticphiqls121 gytvdgtfqh ycianathat iipesvplev aapimcagit cyralkeskv gpgewicipg181 aggglghlav qyakamamrv vaidtgddka elvksfgaev fldfkkeadm ieavkaatng241 gahgtlvlst spksyeqaag farpgstmvt vsmpagaklg adifwltvkm lkicgshvgn301 ridsiealey vsrglvkpyy kvqpfstlpd vyrlmhenki agrivldlsk

Synonyms: ADH1, Adh-1, ADH-AA, Adh-1e, Adh-1t, Adh-3e, Adh1-e, Adh1-t, Adh1tl, Adh3-e, Adh-1-t, AI194826, Adh, Adh1a, ADH, ALCOHOL DEHYDROGENASE, ALCOHOL DEHYDROGENASE 1, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE, ATADH, ATADH1, F22K20.19, F22K20_19, ADC1, DvirAdh, dvir_GLEANR_2771, DvirGJ18208, GJ18208, Adh1, DKFZp470F1127

Application Details

Application Notes: ELISA: 1/5,000-1/25,000). Western Blot: 1/500-1/2,000, expect a band approximately 37 kDa in size corresponding tomonomeric alcohol dehdrogenase in the appropriate cell lysate or extract. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 90.0 mg/ml (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01%(w/v) Sodium Azide as preservative.Reconstitution: Restore with 2.0 ml of deionized water (or equivalent).

Storage: Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. This product is stable for one month at 2-8°C as anundiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and thenaliquot contents and freeze at -20°C or below. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Research Area: Enzymes

Restrictions: For Research Use only

Images

Figure 1. Western blot analysis with Anti-Alcohol Dehydrogenase antibody was used to detect yeast Alcohol Dehydrogenase. Comparison to molecular weight markers (not shown) indicates estimated molecular weights consistent with monomer, dimer and tetramer present in this preparation. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(TM)800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000