Intestinal Alkaline Phosphatase antibody

Catalog No. ABIN116600

The Rabbit Polyclonal anti-Intestinal Alkaline Phosphatase antibody is suitable to detect Intestinal Alkaline Phosphatase in samples from Human. It has been validated for WB and EIA.

Quick Overview for Intestinal Alkaline Phosphatase antibody (ABIN116600)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Intestinal Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Intestinal Alkaline Phosphatase Antibody

Specificity: This antibody detects human Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline phosphatase [human intestine].

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Alkaline Phosphatase [Human Intestine].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of AlkalinePhosphatase [Human Intestine] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassiumphosphate, 0.15 M Sodium chloride, pH 7.2 with 0.01 % (w/v) Sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: Alkaline Phosphatase / ALPI / IAP

Background: Synonyms: Intestinal alkaline phosphatase

Gene ID: 248

NCBI Accession: NP_001622

UniProt: P09923