L-Ascorbate Oxidase antibody
| Antigen |
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| Synonyms |
LOC100281551, LOC100282094, LOC100285297, LOC100285637 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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| Catalog no. |
ABIN116624 |
| Quantity |
2 ml (85.0 mg/ml (by Refractometry)) (Variants) |
| Price |
350.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Ascorbase, ASO, AAO
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Swiss-Prot
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P24792
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Immunogen
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Ascorbate Oxidase [cucurbita].
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Format
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Serum
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Specificity
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Assay by immunoelectrophoresis resulted in a single precipitin arc against purified andpartially purified Ascorbate Oxidase [cucurbita]. Cross reactivity against Ascorbate Oxidasefrom other tissues and species may occur but have not been specifically determined.
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Application Notes
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: : This product has been assayed against 1.0 ug of AscorbateOxidase [cucurbita] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 6,000 to 1: 23,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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85.0 mg/ml (by Refractometry)
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Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%sodium azide as preservative.Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
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Storage
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Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage aliquot contents and freeze at -20°Cor below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.
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Research Area
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Enzymes
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Restrictions
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For Research Use only
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Alternatives for antigen "L-Ascorbate Oxidase", type "Antibodies"
