CA2 antibody

Catalog No. ABIN116661

The Rabbit Polyclonal anti-CA2 antibody (ABIN116661) specifically detects CA2 in WB, IP and EIA. The antibody is reactive with Human and Cow samples.

Quick Overview for CA2 antibody (ABIN116661)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-CA2 Antibody

Specificity: This antibody detects Carbonic anhydrase 2. Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carbonic anhydrase 2 [bovine erythrocytes].

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]

Application Details

Application Notes: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Carbonic Anhydrase II [BovineErythrocytes] in a standard sandwich ELISA using Peroxidase conjugated affinity purifiedanti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 14,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by refractometry)

Buffer: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.

Target Details for CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Alternative Name: Carbonic Anhydrase 2

Background: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II

Gene ID: 280740

NCBI Accession: NP_848667

UniProt: P00921