Choline Oxidase antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Application
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 Alternatives Enzyme Immunoassay (EIA), Western Blotting (WB)
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| Catalog no. |
ABIN116681 |
| Quantity |
2 ml (90.0 mg/ml (by Refractometry)) (Variants) |
| Price |
350.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Swiss-Prot
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P16101
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Immunogen
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Choline Oxidase [Alcaligenes species].
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Format
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Serum
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Description
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Choline Oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine withconcomitant consumption of oxygen and production of hydrogen peroxide.
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Specificity
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Assay by immunoelectrophoresis resulted in a single precipitin arc against purified andpartially purified Choline Oxidase [Alcaligenes species]. Cross reactivity against Choline Oxidase from other sources is unknown.
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Application Notes
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 ug of Choline Oxidase[Alcaligenes species] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Goat IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 32,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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90.0 mg/ml (by Refractometry)
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Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%sodium azide as preservative.Reconstitution: Restore with 2.0 ml of deionized water (or equivalent).
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Storage
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Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear afterstanding at room temperature. For extended storage aliquot contents and freeze at -20°Cor below. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch.
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Research Area
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Signaling, Metabolism, Enzymes
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Restrictions
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For Research Use only
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Alternatives for antigen "Choline Oxidase", type "Antibodies"
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Hosts
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Goat (16), Rabbit (8)
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Reactivities
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Alcaligenes (8)
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Applications
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Western Blotting (WB) (24), ELISA (15), Immunoprecipitation (IP) (9), Immunofluorescence (IF) (8), Enzyme Immunoassay (EIA) (7), Immunohistochemistry (IHC) (4), Dot Blot (Dot) (3), Immunodiffusion (ID) (3), Radioimmunoassay (RIA) (3), Flow Cytometry (FACS) (1)
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Conjugates
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Biotin (5), Texas Red (TR) (4), HRP (3)
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