Cholesterol Oxidase antibody

Catalog No. ABIN116685

The Goat Polyclonal anti-Cholesterol Oxidase antibody (ABIN116685) specifically detects Cholesterol Oxidase in WB and EIA. The antibody is reactive with Bacteria samples.

Quick Overview for Cholesterol Oxidase antibody (ABIN116685)

Target: Cholesterol Oxidase (choS)

Reactivity: Bacteria

Host: Goat

Clonality: Polyclonal

Conjugate: This Cholesterol Oxidase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Cholesterol Oxidase Antibody

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Cholesterol Oxidase [Microorganism].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Cholesterol Oxidase [Microorganism] in astandard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L](Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for30 minutes at room temperature. A working dilution of 1: 8,000 to 1: 40,000 of thereconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for Cholesterol Oxidase

Target: Cholesterol Oxidase (choS)

Alternative Name: Cholesterol Oxidase

Background: Cholesterol Oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology.Synonyms: CHOD, Cholesterol oxidase, EC 1.1.3.6