Glucose-6-Phosphate Dehydrogenase antibody

Catalog No. ABIN116721

The Goat Polyclonal anti-Glucose-6-Phosphate Dehydrogenase antibody (ABIN116721) specifically detects Glucose-6-Phosphate Dehydrogenase in WB and EIA. The antibody is reactive with Leuconostoc samples.

Quick Overview for Glucose-6-Phosphate Dehydrogenase antibody (ABIN116721)

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivity: Leuconostoc

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Glucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Conjugation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg ofGlucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat)and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 8,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 95.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use . Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. This product is stable for one month at 2-8 °C as an undiluted liquid. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature.

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: G6PD

Background: Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase

Gene ID: 1245

UniProt: P11411

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones