Pancreatic Islet Lysate antibody

Antigen: Pancreatic Islet Lysate

Clonality: Polyclonal

Host: Dog

Reactivity: Pig (Porcine)

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN116793

Additional Information

Immunogen: Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells.

Specificity: This product is an IgG fraction antibody purified from monospecific antiserum by amulti-step process which includes delipidation, salt fractionation and ion exchangechromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-DogSerum. Cross reactivity was determined by immunoblot (western) to be minimal against porcinekidney and porcine liver cell lysates.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot) and ELISA. Recommended Dilutions: This product has been assayed against 1.0 ug of Pancreatic IsletLysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. Aworking dilution of 1: 50 to 1: 100 is suggested for this product. This product has beenassayed against 1.5 ug of Pancreatic Islet Lysate [Porcine Pancreas] per lane byImmunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer tonitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG[H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1: 50 to1: 200 is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative.

Storage: Store vial at -70°C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch.

Research Area: Cell/Tissue Markers

Restrictions: For Research Use only

Publications

Publications: Lanza, Jackson, Sullivan et al.: "Xenotransplantation of cells using biodegradable microcapsules." in: Transplantation, Vol. 67, Issue 8, pp. 1105-11, 1999 (PubMed).