Reactivity: E. coli
WB, EIA
Host: Mouse
Monoclonal
8A5-A10
unconjugated
Application Notes
Suitable for Immunoblotting (Western or Dot blot), ELISA and most Immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Penicillinase[Enterobacter cloacae] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti- Rabbit IgG [H&L] (Goat) (R1364HRP) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 6,000 to 1: 26,000 of the reconstitution concentration is suggestedfor this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by Refractometry)
Buffer
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 0.01 % (w/v) sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage
4 °C/-20 °C
Storage Comment
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.