Pepsin antibody

Catalog No. ABIN116802

The Goat Polyclonal anti-Pepsin antibody (ABIN116802) specifically detects Pepsin in WB, IP, EIA, IHC (p) and IHC (fro). The antibody is reactive with Pig samples.

Quick Overview for Pepsin antibody (ABIN116802)

Target: Pepsin

Reactivity: Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Pepsin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Pepsin Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Pepsin [Porcine stomach]. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Pepsin [Porcine stomach].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Pepsin [Porcinestomach] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 11,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Pepsin

Target: Pepsin

Background: Synonyms: PGA, PGA3, PGA4, PGA5, Pepsin A, Pepsinogen A, pepsinogen 3 group I, pepsinogen 4 group I, pepsinogen 5 group I

Gene ID: 396892

NCBI Accession: NP_999038

UniProt: P00791