Transferrin antibody

Catalog No. ABIN116824

This Rabbit Polyclonal antibody specifically detects Transferrin in WB, IP and EIA. It exhibits reactivity toward Human.

Quick Overview for Transferrin antibody (ABIN116824)

Target: Transferrin (TF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Transferrin Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Transferrin [Human Serum]. Cross reactivity against Transferrin from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Transferrin [Human Serum].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA (1: 10,000 to 1: 50,000) andImmunoprecipitation. Recommended Dilutions: This product has been assayed against 1.0 μg of Transferrin[Human Serum] in a standard sandwich ELISA using Peroxidase conjugated Affinity purifiedanti-Rabbit IgG [H&L](Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1: 10,000 to 1: 50,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 80.0 mg/mL (prior to lyophil. by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Transferrin

Target: Transferrin (TF)

Background: Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin

Gene ID: 7018

NCBI Accession: NP_001054

UniProt: P02787

Pathways: Transition Metal Ion Homeostasis