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Rabbit anti-Human IgM (Fc5mu Region) Antibody (TRITC) - Preadsorbed

FACS, IM Polyclonal TRITC
Catalog No. ABIN117300

Quick Overview for Rabbit anti-Human IgM (Fc5mu Region) Antibody (TRITC) - Preadsorbed (ABIN117300)

Target

IgM

Clonality

  • 517
  • 109
Polyclonal

Conjugate

  • 122
  • 84
  • 76
  • 57
  • 33
  • 28
  • 26
  • 23
  • 13
  • 12
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 9
  • 9
  • 9
  • 9
  • 9
  • 7
  • 6
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
TRITC

Application

  • 355
  • 315
  • 305
  • 237
  • 167
  • 115
  • 91
  • 91
  • 64
  • 64
  • 36
  • 35
  • 26
  • 21
  • 21
  • 15
  • 9
  • 6
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunomicroscopy (IM)
  • Binding Specificity

    • 122
    • 93
    • 34
    • 24
    • 10
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Fc5mu Region

    Reactivity

    • 222
    • 165
    • 89
    • 40
    • 30
    • 29
    • 28
    • 22
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 12
    • 4
    • 2
    • 1
    Human

    Host

    • 353
    • 220
    • 97
    • 25
    • 14
    • 5
    • 4
    • 1
    • 1
    Rabbit

    Characteristics

    Molar Ratio: 3.5 moles TRITC per mole of Goat IgG

    Purification

    Preadsorption: Solid phase absorption

    Immunogen

    Human IgM (Fc5µ) fragment
  • Application Notes

    Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency.

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.

    Comment

    Absorption / Emission: 550 nm / 570 nm

    Restrictions

    For Research Use only
  • Reconstitution

    Restore with 1.0 mL of deionized water (or equivalent).

    Concentration

    1.5 mg/mL

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Centrifuge product if not completely clear after standing at room temperature.
    Product is photosensitive and should be protected from light.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
  • Target

    IgM

    Target Type

    Antibody

    Background

    Synonyms: Human Immunoglobulin M
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