CD180 antibody

Catalog No. ABIN1176856

Product Details anti-CD180 Antibody

Target: CD180 (CD180 Molecule (CD180))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD180 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: BALB/c mouse splenocytes

Clone: RP-14

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD180 antibody (ABIN1176856)

Ogata, Su, Miyake, Nagai, Akashi, Mecklenbräuker, Rajewsky, Kimoto, Tarakhovsky: "The toll-like receptor protein RP105 regulates lipopolysaccharide signaling in B cells." in: The Journal of experimental medicine, Vol. 192, Issue 1, pp. 23-9, (2000) (PubMed).

Corcoran, Metcalf: "IL-5 and Rp105 signaling defects in B cells from commonly used 129 mouse substrains." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 11, pp. 5836-42, (1999) (PubMed).

Chan, Mecklenbräuker, Su, Texido, Leitges, Carsetti, Lowell, Rajewsky, Miyake, Tarakhovsky: "The molecular mechanism of B cell activation by toll-like receptor protein RP-105." in: The Journal of experimental medicine, Vol. 188, Issue 1, pp. 93-101, (1998) (PubMed).

Miyake, Shimazu, Kondo, Niki, Akashi, Ogata, Yamashita, Miura, Kimoto: "Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1348-53, (1998) (PubMed).

Yamashita, Miyake, Miura, Kaneko, Yagita, Suda, Nagata, Nomura, Sakaguchi, Kimoto: "Activation mediated by RP105 but not CD40 makes normal B cells susceptible to anti-IgM-induced apoptosis: a role for Fc receptor coligation." in: The Journal of experimental medicine, Vol. 184, Issue 1, pp. 113-20, (1996) (PubMed).

Miyake, Yamashita, Ogata, Sudo, Kimoto: "RP105, a novel B cell surface molecule implicated in B cell activation, is a member of the leucine-rich repeat protein family." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3333-40, (1995) (PubMed).

Miyake, Yamashita, Hitoshi, Takatsu, Kimoto: "Murine B cell proliferation and protection from apoptosis with an antibody against a 105-kD molecule: unresponsiveness of X-linked immunodeficient B cells." in: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1217-24, (1994) (PubMed).

Target Details for CD180

Target: CD180 (CD180 Molecule (CD180))

Alternative Name: CD180 (CD180 Products)

Background: The RP/14 antibody reacts with CD180, the radioprotective 105 kDa (RP105) antigen expressed on mature B lymphocytes. Like other members of the Toll-like Receptor (TLR) family, the extracellular region of CD180 contains tandem repeats of a leucine-rich motif flanked by regions containing conserved cysteines. These characteristic structural components of TLR may mediate protein-protein interactions and regulation of signal transduction involved in innate immune responses. Furthermore, CD180 associates with the secretory protein MD-1 on the cell surface. Cross-linking of cell-surface CD180 with the RP/14 antibody induces activation signals in B lymphocytes. Despite the expression of CD180 on lymphocytes of all strains tested (BALB/c, BALB/c.xid, C3H, C57BL/6, CBA/N, and 129/Sv), some strains (BALB/c.xid, C57BL/6.xid, CBA/N, 129/Sv, and 129/Ola) display deficient responses to that stimulatory effects of mAb RP/14.
Synonyms: Ly78, Ly-78, Lymphocyte antigen 78, RP105, Radioprotective 105 kDa protein

Pathways: Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades