PDGFRB antibody (pTyr1021) (Alexa Fluor 488)

Catalog No. ABIN1177129

This Mouse Monoclonal antibody specifically detects PDGFRB in ICS. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for PDGFRB antibody (pTyr1021) (Alexa Fluor 488) (ABIN1177129)

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDGFRB antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: J105-412

Product Details anti-PDGFRB Antibody (Alexa Fluor 488)

Binding Specificity: pTyr1021

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human PDGFRbeta (pY1021)

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-PDGFRB antibody (Alexa Fluor 488) (ABIN1177129)

Claesson-Welsh: "Platelet-derived growth factor receptor signals." in: The Journal of biological chemistry, Vol. 269, Issue 51, pp. 32023-6, (1995) (PubMed).

Target Details for PDGFRB

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Background: Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and exerts its effects by binding to the PDGF receptor (PDGFR), a transmembrane protein tyrosine kinase. PDGFR is composed of PDGFRalpha (CD140a) and/or PDGFRbeta (CD140b) polypeptides. Both PDGF and PDGFR consist of subunits that form homo- or heterodimers with varying specificities: PDGF-AA binds only to alphaalpha PDGFR, PDGF-AB binds to both alphaalpha and alphabeta PDGFR, and PDGF-BB binds to all three PDGFRs. Ligand binding induces dimerization and activation of the receptor. Upon activation, CD140b is phosphorylated at multiple tyrosine sites and, in turn, an intracellular phosphorylation cascade is initiated. PDGFR localizes primarily to membrane invaginations termed caveolae, compartments that are enriched in several of its downstream effectors, including phosphatidylinositol 3'-kinase, Src, and phospholipase C-gamma (PLC-gamma). The J105-412 monoclonal antibody recognizes the phosphorylated tyrosine 1021 (pY1021) in the C-terminal noncatalytic region of CD140b, which interacts primarily with PLC-gamma. The orthologous phosphorylation site in mouse PDGFRbeta is Y1020.

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling