RAD9A antibody (pSer1260)

Catalog No. ABIN117921

The Rabbit Polyclonal anti-RAD9A antibody (ABIN117921) specifically detects RAD9A in WB and EIA. The antibody is reactive with Saccharomyces cerevisiae samples.

Quick Overview for RAD9A antibody (pSer1260) (ABIN117921)

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Reactivity: Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD9A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RAD9A Antibody

Binding Specificity: AA 1249-1263, pSer1260

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to phosphorylated form of aa 1249-1263 of 1309 of yeast Rad9 protein conjugated to KLH.

Application Details

Application Notes: This phospho specific polyclonal antibody was tested by Immunoblotting and ELISA. Datafrom both Immunoblotting and ELISA indicate the antibody is reactive with thephosphorylated form of the immunizing peptide and minimally reactive with thenon-phosphorylated form of the immunizing peptide. Immunoblotting detects yeast Rad9protein. No reactivity is expected against the human or mouse analogs of RAD9. Reactivityagainst RAD9 from other sources is unknown. Cross reactivity may occur withauto-phosphorylated Rad53 kinase. Although not tested, this antibody is likely functionalby IHC and IP. Recommended Dilution(s): This product has been assayed against 0.1 g ofphosphorylated peptide (pS1260) in a standard capture ELISA using TMB(3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature. Aworking dilution of 1: 5,000 is suggested for this product. Minimal reactivity was detectedagainst the nonphosphorylated form (S1260) of the immunizing peptide. This antibodyappears to be specific for the active form (phosphorylated) of the protein. Dilute theantibody 1: 100 to 1: 500 for immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.41 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for RAD9A

Target: RAD9A (RAD9 Homolog A (S. Pombe) (RAD9A))

Alternative Name: RAD9A

Background: Rad9 is required for the MEC1/TEL1-dependent activation of Saccharomyces cerevisiae DNA damage checkpoint pathways mediated by Rad53 and Chk1. DNA damage induces Rad9 phosphorylation, and Rad53 specifically associates with phosphorylated Rad9. Cells have evolved multiple strategies for tolerating genomic damage. The most important of these are numerous repair systems that remove or bypass potentially mutagenic DNA lesions. Another cellular strategy is to delay cell-cycle transitions at multiple points. The genetic control of these delays, termed `checkpoints', was first established in budding yeast where it was shown that the RAD9 gene functions in G2/M arrest after irradiation with X-rays. Subsequently, it has become clear that Rad9 also functions at the G1/S,intra-S and mid-anaphase checkpoints. Defects in checkpoint regulation can lead to genome instability and, in higher eukaryotes, neoplastic transformation. Rad9 also controls the transcriptional induction of a DNA damage regulon (DDR). Rad9 may also have a pro-apoptotic function. This is suggested in that Rad9 from Schizosaccharomyces pombe (SpRad9) contains a group of amino acids with similarity to the Bcl-2 homology 3 death domain, which is required for SpRad9 interaction with human Bcl-2 and apoptosis induction in human cells. Overexpression of Bcl-2 in S. pombe inhibits cell growth independently of rad9, but enhances resistance of rad9-null cells to methyl methanesulfonate, ultraviolet and ionizing radiation. Rad9 conveys the checkpoint signal by activating Rad53p and Chk1p, is hyperphosphorylated by Mec1p and Tel1p, and is a potential Cdc28p substrate. Mature yeast Rad9 is reported to have an apparent molecular weight of ~148 kDa. The human homolog is reported at 48.5 kDa.Synonyms: Cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC=3.1.11.2, RAD-9A

NCBI Accession: NP_004575

UniProt: Q99638

Pathways: Positive Regulation of Response to DNA Damage Stimulus