DCLRE1C antibody (AA 482-495)

Catalog No. ABIN117935

The Goat Polyclonal anti-DCLRE1C antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect DCLRE1C in samples from Human.

Quick Overview for DCLRE1C antibody (AA 482-495) (ABIN117935)

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DCLRE1C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-DCLRE1C Antibody

Binding Specificity: AA 482-495

Specificity: This antibody detects a band of the appropriate size in a number of different cell lines. However, it does not detect a band in cell lines that do not express ARTEMIS, such as the cell line CJ and in an hTERT line.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.

Immunogen: This antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 482-495 of Human ARTEMIS (DCLRE1C DNA cross- link repair 1C). Genename: DCLRE1C

Application Details

Application Notes: This antibody has been tested for use in ELISA (1: 10,000-1: 20,000), Immunohistochemistry(1: 1,000) and Western blotting (1: 500-1: 2,000). Reactivity in other immunoassays isunknown. This antibody detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.2 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, with 0.01 %(w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for DCLRE1C

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Alternative Name: Artemis

Background: Artemis has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. DNAPKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in non-homologous DNA end joining.Synonyms: A-SCID protein, ASCID, DCLRE1C, DNA cross-link repair 1C protein, SCIDA, SNM1-like protein, SNM1C

Gene ID: 64421

NCBI Accession: NP_001029027

UniProt: Q96SD1

Pathways: DNA Damage Repair