CAND1 antibody (C-Term)

Catalog No. ABIN117945

This anti-CAND1 antibody is a Rabbit Polyclonal antibody detecting CAND1 in WB, IP and EIA. Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for CAND1 antibody (C-Term) (ABIN117945)

Target: CAND1 (Cullin-Associated and Neddylation-Dissociated 1 (CAND1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAND1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-CAND1 Antibody

Binding Specificity: AA 1130-1143, C-Term

Sequence: Human CAND2 (TIP120B) (1143 aa 125.4 kDa): 1 mstaafhiss llekmtssdk dfrfmatsdl mselqkdsiq ldedserkvv kmllrlledk 61 ngevqnlavk wlgvplgafh asllhcllpq lssprlavrk ravgalghla aacstdlfve 121 ladhlldrlp gprvptspta irtliqclgs vgrqaghrlg ahldrlvplv edfcnlddde 181 lresclqafe aflrkcpkem gphvpnvtsl clqyikhdpn ynydsdedee qmetedsefs 241 eqesedeysd dddmswkvrr aaakciaali ssrpdllpdf hctlapvlir rfkereenvk --- 901 vkflisdqph pidpllksfi gefmeslqdp dlnvrratla ffnsavhnkp slvrdllddi 961 lpllyqetki rrdlirevem gpfkhtvddg ldvrkaafec mysllesclg qldiceflnh 1021 vedglkdhyd irmltfimva rlatlcpapv lqrvdrliep lratctakvk agsvkqefek 1081 qdelkrsamr avaalltipe vgkspimadf ssqirsnpel aalfesiqkd sasapstdsm 1141 els

Specificity: This antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. It reacts with human, rat and mouse CAND2/TIP120B. Cross reactivity does occur with human, rat and mouse CAND1/TIP120A. Cross reactivity with CAND2 from other sources is not known.

Purification: Delipidation and defibrination.

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH.

Application Details

Application Notes: Western blot (1: 500-1: 1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from transfected cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been tested. A 125.4 kDa bandcorresponding to human CAND2 is detected. CAND2 is specifically expressed in muscle

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CAND1 antibody (ABIN117945)

Concejero, Chen, Wang, Wang, Lin, Liu, Yang, Yong, Lin, Jawan, Huang, Cheng, Eng: "Living donor liver transplantation for hepatocellular carcinoma: a single-center experience in Taiwan." in: Transplantation, Vol. 85, Issue 3, pp. 398-406, (2008) (PubMed).

Target Details for CAND1

Target: CAND1 (Cullin-Associated and Neddylation-Dissociated 1 (CAND1))

Alternative Name: CAND1

Background: CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60 % identical in amino acid sequence to TIP120A.Synonyms: Cullin-associated NEDD8-dissociated protein 1, Cullin-associated and neddylation-dissociated protein 1, KIAA0829, TBP-interacting protein TIP120A, TBP-interacting protein of 120 kDa A, TIP120, TIP120A, p120 CAND1

Gene ID: 55832

NCBI Accession: NP_060918

UniProt: Q86VP6

Pathways: Regulation of Actin Filament Polymerization