Cullin 3 (CUL3) antibody

Antigen: Cullin 3 (CUL3)

Synonyms: CUL-3, Cullin-3, cul3, cul3a, CUL-3-A, MGC79108, Cullin-3-A, cul3b, CUL-3-B, MGC80402, Cullin-3-B, AI467304, AW146203, mKIAA0617, MGC189292, wu:fc37e01, CUL3, ARABIDOPSIS THALIANA CULLIN 3, ARABIDOPSIS THALIANA CULLIN 3A, ATCUL3, ATCUL3A, CUL3A, CULLIN 3, CULLIN 3A, T24P13.25

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: ELISA, Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN117949

Additional Information

Alternative name: Cullin-3

UniProt: Q13618

Immunogen: Synthetic peptide corresponding to amino acids 1-12 of Human Cul3 (N-terminus) coupledto KLH. AA Sequence: Human Cullin 3 (768 aa 88. 9 kDa): 1 msnlskgtgs rkdtkmrira fpmtmdekyv nsiwdllkna iqeiqrknns glsfeelyrn61 aytmvlhkhg eklytglrev vtehlinkvr edvlnslnnn flqtlnqawn dhqtamvmir121 dilmymdrvy vqqnnvenvy nlgliifrdq vvrygcirdh lrqtlldmia rerkgevvdr181 gairnacqml milglegrsv yeedfeapfl emsaeffqme sqkflaensa svyikkvear241 ineeiervmh cldksteepi vkvverelis khmktiveme nsglvhmlkn gktedlgcmy301 klfsrvpngl ktmcecmssy lreqgkalvs eegegknpvd yiqglldlks rfdrfllesf361 nndrlfkqti agdfeyflnl nsrspeylsl fiddklkkgv kglteqevet ildkamvlfr421 fmqekdvfer yykqhlarrl ltnksvsdds eknmisklkt ecgcqftskl egmfrdmsis481 nttmdefrqh lqatgvslgg vdltvrvltt gywptqsatp kcnippaprh afeifrrfyl541 akhsgrqltl qhhmgsadln atfygpvkke dgsevgvgga qvtgsntrkh ilqvstfqmt601 ilmlfnnrek ytfeeiqqet diperelvra lqslacgkpt qrvltkepks keienghift661 vndqftsklh rvkiqtvaak qgesdperke trqkvdddrk heieaaivri mksrkkmqhn721 vlvaevtqql karflpspvv ikkrieglie reylartped rkvytyva

Cross-Reactivity: Human, Mouse (Murine)

Description: Cullins assemble a potentially large number of ubiquitin ligases by binding to the RINGprotein ROC1 to catalyse polyubiquitination, as well as binding to various specificityfactors to recruit substrates. Cullin 3 is an essential component of the SCF(SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitinationof proteins involved in cell cycle progression, signal transduction and transcription. In theSCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1subunits. Cul3 may also contribute to catalysis through positioning of the substrate andthe ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of theSCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and ispart of a complex with TIP120A/CAND1, Cyclin E and RBX1.

Characteristics: Synonyms: CUL-3, CUL3, KIAA0617

Synonyms: CUL-3, Cullin-3, cul3, cul3a, CUL-3-A, MGC79108, Cullin-3-A, cul3b, CUL-3-B, MGC80402, Cullin-3-B, AI467304, AW146203, mKIAA0617, MGC189292, wu:fc37e01, CUL3, ARABIDOPSIS THALIANA CULLIN 3, ARABIDOPSIS THALIANA CULLIN 3A, ATCUL3, ATCUL3A, CUL3A, CULLIN 3, CULLIN 3A, T24P13.25

Application Details

Application Notes: Western blot: This antibody reacts with human Cul3 (1/500-1/1,000, An 88.9 kDa bandcorresponding to human Cul3 is detected, Most cell lines expressing Cul3 can be used as apositive control).

Concentration: 85.0 mg/ml (by Refractometry)

Purification: Serum

Storage: Store vial at -20°C prior to opening. Centrifuge product if not completely clear afterstanding at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.

Publications

Furukawa, He, Borchers et al.: "Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases." in: Nature cell biology, Vol. 5, Issue 11, pp. 1001-7, 2003 (PubMed).

Chuang, Huang, Hsieh et al.: "Sporadic paediatric and adult Burkitt lymphomas share similar phenotypic and genotypic features." in: Histopathology, Vol. 52, Issue 4, pp. 427-35, 2008 (PubMed).