SKP1 antibody (C-Term)

Catalog No. ABIN117959

The Rabbit Polyclonal anti-SKP1 antibody has been validated for WB, IP and EIA. It is suitable to detect SKP1 in samples from Human. There is 1 publication available.

Quick Overview for SKP1 antibody (C-Term) (ABIN117959)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SKP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-SKP1 Antibody

Binding Specificity: AA 152-163, C-Term

Sequence: Human SKP1 (163 aa 18.5 kDa) 1 mpsiklqssd geifevdvei akqsvtiktm ledlgmddeg dddpvplpnv naailkkviq 61 wcthhkddpp ppeddenkek rtddipvwdq eflkvdqgtl felilaanyl dikglldvtc 121 ktvanmikgk tpeeirktfn ikndfteeee aqvrkenqwc eek

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human SKP1. Cross reactivity is expected against mouse SKP1 based on a high degree of sequence homology. Cross reactivity with other SKP1 proteins or SKP1 from other sources is not known.

Purification: Delipidation and defibrination.

Immunogen: Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH.

Application Details

Application Notes: ELISA (1/2,000-1/10,000). This antibody reacts with human SKP1 by Western blot(1/500-1/1,000) and Immunoprecipitation. The antibody Immunoprecipitates in vitrotranslated protein and protein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation of cyclin A and Cuol1 may occur. An 18.5 kDa band correspondingto human SKP1 is detected. Most cell lines expressing SKP1 can be used as a positivecontrol.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85.0 mg/mL (by Refractometry)

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

References for anti-SKP1 antibody (ABIN117959)

Wang, He, Wang, Wang, Zhai, Zhou, Zhang, Zhai, Feng, Han: "[Impact of Donor KIR2DS5 Genotype on Outcome Following Haploidentical Hematopoietic Stem Cell Transplantation.]" in: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 1, pp. 111-5, (2008) (PubMed).

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1 / OCP2

Background: SKP1 is also known as S-phase kinase-associated protein 1A, Cyclin A/CDK2-associated protein p19, p19A, p19skp1, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, OCP-II protein, OCP-2, Transcription elongation factor B, and SIII. SKP1 is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, SKP1 serves as an adapter that links the F-box protein to CUL1. SKP1 interacts directly with CUL1 and F-box proteins, such as BTRC and SKP2, in the SCF complex. SKP1 also interacts with FBX29 and the cyclin A/CDK2 complex. SKP1 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8 and is also a component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X.Synonyms: Cyclin A/CDK2-associated protein p19, EMC19, OCP-2, OCP-II, Organ of Corti protein 2, RNA polymerase II elongation factor-like protein, S-phase kinase-associated protein 1, SKP1A, TCEB1L, Transcription elongation factor B, p19A, p19skp1

Gene ID: 6500

NCBI Accession: NP_008861

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases