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Invertase antibody

Antigen

Invertase

Clonality Polyclonal
Host
Application
Alternatives Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
Catalog no. ABIN117966
Quantity 2.0 ml  (90.0 mg/ml (by UV absorbance at 280...)  (Variants)
Price 350.00 $   Plus shipping costs $35.00
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Availability Ships within 7 to 10 Business Days

Additional Information

Characteristics Synonyms: Beta-fructofuranosidase, Saccharase, INV1
Gene ID 2896726
Swiss-Prot Q9Y746
Immunogen Invertase [Candida]
Format Serum
Description Invertase hydrolyses the terminal non reducing beta D fructofuranoside residues in beta Dfructofuranosides. It is the enzyme that bees use to convert nectar into honey. Industrialand confectionary applications use yeast invertase to split sucrose into fructose andglucose and also to improve the shelf life of food products.
Specificity This product was prepared from monospecific antiserum. Assay by immunoelectrophoresisresulted in a single precipitin arc against purified and partially purified Invertase[Candida]. Cross reactivity against Invertase from other sources is unknown.

Application Details

Application Notes Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 ug of Invertase [Candida] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 10,000 to 1: 65,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 90.0 mg/ml (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative.Reconstitution: Restore with 2.0 ml of deionized water (or equivalent).
Storage Store vial at 2-8°C prior to restoration. Restore with 2. 0 ml of deionized water (orequivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior toimmediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.
Research Area Enzymes, Metabolism
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Invertase", type "Antibodies"
Hosts Goat (16)
Reactivities Candida (4)
Applications Western Blotting (WB) (16), ELISA (12), Immunoprecipitation (IP) (10), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (IHC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunofluorescence (IF) (1)
Conjugates Texas Red (TR) (4), Biotin (3), HRP (3)