Cecropin B antibody

Catalog No. ABIN117971

The Rabbit Polyclonal anti-Cecropin B antibody is suitable to detect Cecropin B in samples from Bombyx mori. It has been validated for IP, WB and EIA.

Quick Overview for Cecropin B antibody (ABIN117971)

Target: Cecropin B

Reactivity: Bombyx mori

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cecropin B antibody is un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Cecropin B Antibody

Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using cecropin B coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified cecropin B. Reactivity against other forms of cecropins has not yet been determined.

Purification: Immunoaffinity chromatography

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetically produced fulllength cecropin B corresponding to a modified sequence from that which is found in silkworm: Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Val-Gly-Arg-Asn- Ile-Arg-Asn-Gly- Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala- Lys-Ala-Leu

Application Details

Application Notes: Anti-cecropin B is useful for the detection of cecropin B in various immunological assays. This reagent has specific value to study cecropins in transgenic plants. The antibody mayalso recognize naturally occurring cecropin A. Cecropin B has been modified in someinstances by substituting a single amino acid to render the molecule less susceptible toproteolytic cleavage. This reagent is suitable for the detection of cecropin B inImmunoblotting (1: 1000), Immunoprecipitation and ELISA experiments. A single 4400dalton band is detected in protein extracts.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.3 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.01 M Potassium Phosphate, 0.075 M Sodium Chloride, pH 7.2 with 50 % (v/v) Glycerol as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Target Details for Cecropin B

Target: Cecropin B

Alternative Name: Cecropin-B

Background: Cecropins are produced by insects, particularly under conditions of infection. Cecropins are bioactive peptides that exhibit activities by interacting with membranes and forming transmembrane channels that allow the free flow of electrolytes, metabolites and water across the phospholipid bilayers. Cecropins A, B and D are close homologues consisting of 35-39 residues. They are found in the pupae of the cecropin moth, but related homologues named lepodopteran, bactericidin, moricin and sarcotoxin are produced by other insects.Synonyms: CECB1, Lepidopteran-A/B

Gene ID: 693028

NCBI Accession: NP_001037460

UniProt: P04142