Myeloperoxidase antibody

Catalog No. ABIN117976

This Rabbit Polyclonal antibody specifically detects Myeloperoxidase in WB, IP and EIA. It exhibits reactivity toward Human.

Quick Overview for Myeloperoxidase antibody (ABIN117976)

Target: Myeloperoxidase (MPO)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myeloperoxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Myeloperoxidase Antibody

Purification: Multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below.

Immunogen: Myeloperoxidase [Human Leukocytes]

Application Details

Application Notes: This antibody is suitable for western blotting, ELISA (1: 200,000) and Immunoprecipitation. Although not tested, this antibody is likely functional in Immunohistochemistry and otherimmunological methods. This product was assayed by Immunoblot and was found to bereactive with human myeloperoxidase isolated from leukocytes. The blot was probed withImmunochemical's anti-Myeloperoxidase [Human Leukocytes] used at a dilution of 1: 2000. Use Peroxidase Conjugated Affinity Purified Anti-RABBIT IgG (H&L) (GOAT) as a secondaryantibody for detection. The antibody detects a multiple bands corresponding to 53 kDa and15 kDa polypeptides and possibly other chain combination (68 kDa and 106 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Gentamicin Sulfate as preservative.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at RT. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Myeloperoxidase

Target: Myeloperoxidase (MPO)

Alternative Name: Myeloperoxidase

Background: Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5 % . MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.Synonyms: MPO

Gene ID: 9606

UniProt: P05164

Pathways: Chromatin Binding