Nestin antibody (AA 1484-1500)

Catalog No. ABIN117995

The Rabbit Polyclonal anti-Nestin antibody is suitable to detect Nestin in samples from Human and Monkey. It has been validated for WB and EIA.

Quick Overview for Nestin antibody (AA 1484-1500) (ABIN117995)

Target: Nestin (NES)

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Nestin Antibody

Binding Specificity: AA 1484-1500

Specificity: This antibody detects Nestin protein.

Cross-Reactivity (Details): Species reactivity (expected):A BLAST analysis was used to suggest cross reactivity with Nestin protein from Human and Macaque (100 % homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from Rabbit (94 %), Mouse (reactivity shown above), Rat and Hamster (88 %), Bovine and Dog (82 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: A recombinant protein corresponding to amino acids 1484-1500 of Human Nestin protein.

Application Details

Application Notes: This antibody has been tested for use in ELISA and Western blot. Expect a bandapproximately 200-220 kDa in size corresponding to Nestin protein by Western blotting inthe appropriate cell lysate or extract. Recommended Dilutions: ELISA: 1/20,000-1/80,000. Western blot: 1/500-1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Nestin is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.Synonyms: NES, Nbla00170

Gene ID: 10763

NCBI Accession: NP_006608

UniProt: P48681