LIM Domain Binding 2 (LDB2) antibody

Antigen: LIM Domain Binding 2 (LDB2)

Synonyms: LDB1, CLIM1, Ldb3, CLP-36, AI035351, AW146358, LDB2, CLIM-1, MGC127025

Clonality: Polyclonal

Host: Rabbit

Reactivity: Chicken, Human, Mouse (Murine)

Application: ELISA

Catalog no.: ABIN118016

Additional Information

Alternative name: LDB2

UniProt: O55203

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeatedimmunizations with a synthetic peptide corresponding to aa 107-120 of Mouse LDB2. AA Sequence: S-K-E-S-Y-H-N-S-S-I-T-V-D

Cross-Reactivity: Chicken, Human, Mouse (Murine)

Description: LDB2 (LIM homeobox protein cofactor CLIM-1a). It is suggested that LIM domain bindingproteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency byacting as co-factors for LIM homeodomain and Otx class transcription factors both of whichhave essential roles in development. LDB2 is a close homologue of LDB1. LDBs arehomologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1,Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95%, 73% and 62% amino acididentity with mouse Ldb1, respectively.

Characteristics: Synonyms: LDB2, CLIM1, LIM domain-binding protein 2, Carboxyl-terminal LIM domain-binding protein1, CLIM-1, LIM domain-binding factor CLIM1

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 5,000-1: 20,000)andWestern Blot (1: 500-1: 2,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 43 kDa in size corresponding to LDB2 by westernblotting in the appropriate cell lysate or extract. Immunofluorescence microscopy. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.65 mg/ml (by UV absorbance at 280 nm)

Purification: Affinity Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative.

Storage: Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

Figure 1. Western blot using Affinity Purified anti-LDB2 antibody shows detection of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Publications

Semina, Altherr, Murray: "Cloning and chromosomal localization of two novel human genes encoding LIM-domain binding factors CLIM1 and CLIM2/LDB1/NLI." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 9, Issue 11, pp. 921-4, 1998 (PubMed).

Ostendorff, Peirano, Peters et al.: "Ubiquitination-dependent cofactor exchange on LIM homeodomain transcription factors." in: Nature, Vol. 416, Issue 6876, pp. 99-103, 2002 (PubMed).

Shimizu, Shimizu, Kinebuchi et al.: "[A case of acute eosinophilic pneumonia probably induced by minocycline]" in: Nihon Koky?ki Gakkai zasshi = the journal of the Japanese Respiratory Society, Vol. 46, Issue 2, pp. 136-40, 2008 (PubMed).