Thyroglobulin antibody

Catalog No. ABIN118049

The Mouse Monoclonal anti-Thyroglobulin antibody is suitable to detect Thyroglobulin in samples from Human, Mouse, Rat and Dog. It has been validated for WB, ELISA, IHC (p), IP and IHC (fro).

Quick Overview for Thyroglobulin antibody (ABIN118049)

Target: Thyroglobulin (TG)

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thyroglobulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2H11

Product Details anti-Thyroglobulin Antibody

Specificity: This protein A purified mouse monoclonal antibody reacts specifically with thyroglobulin in human tissues. The antibody recognizes a 330-kDa band corresponding to thyroglobulin. Cross reactivity with thyroglobulin from mouse, rat and dog will occur. Cross reactivity with thyroglobulin from other sources has not been determined.

Purification: Protein A chromatography.

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with human thyroglobulin protein. Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma SP2/0 cells after immunization with thyroglobulin from human thyroid gland using conventional hybridoma technology.

Isotype: IgG1

Application Details

Application Notes: Suitable for the detection of thyroglobulin in ELISA, Immunohistochemistry,Immunoblotting and Immunoprecipitation. For Immunohistochemistry, both frozen sections and formalin fixed, paraffin-embeddedtissue sections can be used without epitope retrieval or enzyme digestion. This antibody isspecific for the 330 kDa thyroglobulin protein. Thyroglobulin shows a cytoplasmiclocalization. Thyroid tissue can be used as a positive control. Generally a 1: 50 to 1: 200dilution is suitable for most applications. A 30 to 60 minute incubation time is usuallysufficient. However, depending upon the fixation conditions and the staining systememployed, optimal incubation should be determined by the user. Both frozen sections andformalin fixed, paraffin embedded tissues are recommended for preparations to use forImmunohistochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.6 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8°C.

Expiry Date: 12 months

Target Details for Thyroglobulin

Target: Thyroglobulin (TG)

Background: Thyroglobulin (Tg) is synthesized by the follicular epithelial cells of the thyroid and secreted from the thyroid gland with the stimulation of TSH and/or thyroid stimulating immunoglobulins. Thyroglobulin is a prognostic marker for Graves` disease. Thyroglobulin antibody has been useful in the positive identification of thyroid carcinomas of the papillary and follicular types.
Alternate names: TGN, Thyreoglobulin

Gene ID: 7038

NCBI Accession: NP_003226

UniProt: P01266

Pathways: Thyroid Hormone Synthesis